SBNet

SBNet - Travel Reports

Students who have been awarded a foreign travel grant are expected to:
  1. write a short report of their trip (in HTML). These reports will be made available through this page. (Use this form to submit your travel report !)

  2. be prepared to travel to other interested laboratories in Sweden to give a presentation about the results of their trip (travel cost associated with such trips will be covered by the Network, separately from the foreign travel award).

The following table shows details of awarded travel grants. If you are a Swedish structural biology PI and would like to invite any of the students to your laboratory to give a presentation about their trip, you can send them an E-mail (click on the name of the student). Click on the "purpose" of the trip to read a brief travel report written by the student.

| 2003 (28) | 2002 (12) | 2001 (15) | 2000 (4) | 1999 (4) | 1998 (4) | 1997 (1) |

2003

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(68) Linda Öster 7 November, 2003 Attend the practical course M2M-3 at the EMBL Hamburg Outstation 6-13 November, 2003
This course is aimed for PhD-students in their last year and will cover a wide range of topics in macromolecular crystallography that I will have use of. There will both be lectures, beamline practicals and computational tutorials held by the experts in the field.
(67) Anna Jansson (KI) 20 October, 2003 Visit collaborating laboratory at University of Turku. 29 November - 20 December, 2003
I'm doing my PhD in the field Structural Biology and the main focus of my PhD studies is to use crystallography as a tool for studying proteins alone and in complex with substrates/inhibitors and to by additional methods as mutagenisis and biochemical analysis elucidate their functions. In my project I work with some proteins involved in the production of anthracyclines, natural products commonly used in cancer treatment. Many of the anthracyclines have however been shown to exhert cardiotoxic sideffects and it is therefore important to find new potential drugs. Novel antibiotics have been produced by transferring genes between different Streptomyces species and I am studying enzymes responsible for the production of some of these new products. I have recently been solving the structures of two of these enzymes and the results have recently been published in three papers (see project description, publications). I am having less then one year left of my PhD studies and the last project that I am doing is involving biochemical studies of one of the enzymes, RdmB. These measurements would have to been done in Finland and therefore I hope that I can get the chance to go there in the end of this year to get the results I need to write up my thesis. The three dimensional structure of RdmB has the fold of a methyltransferase but the enzyme actually works as a hydroxylase adding a hydroxylgroup to the anthracycline ring structure. The research to be done in Turku would include elucidating if the oxygen in the added hydroxylgroup of the substate comes from molecular oxygen or water. I will also test if the substrate reacts via a sulfenic cystein intermediate, which might show that the enzyme is working similar to a peroxidase mechanism. I also want to go to Finland to get some insight into how it is working in another lab and study the production of the Streptomyces enzymes I have been provided.
(66) Johan Sagemark (KI) 10 October, 2003 Attend a Varian NMR user course in Darmstadt, Germany 11-15 November, 2003
NMR is a central technique in my research. We have recently installed Varian electronics to our NMR magnet. A course how to operate the new system efficiently is necessary. This knowledge will boost my research since I have several projects waiting.
(65) Malin Uppsten (SLU) 10 October, 2003 Attend the practical course on training in methods for macromolecular crystallography M2M-3: From Measurement to Model. The course will take place at the EMBL Hamburg Outstation on the DESY synchrotron. 6-13 November, 2003
I am in my last year as a graduate student in x-ray crystallography. During the years I have been working with several projects concerning ribonucleotide reductases (RNRs). Since the first RNR structures were solved about ten years ago, the phasing method I have been focusing is molecular replacement.
(64) Anna-Karin Lundbäck (KI) 29 September, 2003 Attend "Biophysical studies of membrane proteins" (workshop), Strassbourg 14-17 October, 2003 14 - 17 October, 2003
I would like to attend this workshop called "Biophysical studies of membrane proteins", which is organized by INSERM. The main focus of the workshop will be on methods to study membrane proteins. The course will introduce a wide variety of techniques for expression, purification, reconstitution into lipid bilayers, 3D an 2D crystallization and characterization.
(63) Ulrika Schagerlöf (Lund University) 25 June, 2003 Attend the CSF Workshop "Structure, dynamics and function of membrane proteins" in Ascona 5-10 October, 2003
I have been working with membrane proteins for more than one year and during this time I have accumulated a certain amount of practical experience and theoretical knowledge. It would be very valuable for me at this point in my work to be able to meet with other people in the same field and exchange thoughts and experiences. It would also give me an opportunity to present my own work and get valuable input in my further research and keep me informed of the current status of my research field.
(62) Patrik Lundström (Lund University) 1 September, 2003 Attend the 2nd International Summer School in Solid State NMR Spectroscopy, Niederöblarn, Austria 6 - 12 September, 2003
Solid state NMR is predicted to be an increasingly more important technique in structural biology. It circumvents many of the difficulties of both X-ray crystallography and liquid state NMR, i.e. non- crystalline samples and proteins with low solubility may be studied. An obvoius future application is structural characterization of membrane proteins. The theory is however very complex and involves several features which never enters liquid state NMR. Solid state NMR spectroscopy is currently not practiced by many Swedish scientists and it would be difficult to learn the material covered in the workshop by someone in Sweden or by myself. With this workshop, guided by expert teachers, I will learn about the full potential of biomolecular solid state NMR spectroscopy and get the necessary theoretical framework to understand the details. This would be unique competence to the Swedish structural biology community.
(61) Anders Pedersen (Gothenburg University) 25 June, 2003 Attend the EuroLab Course "Advanced Computing in NMR Spectroscopy" 30 August - 6 September, 2003
The topics are spot-on with regards to my present and future work on two fairly large proteins(20.4 kDa and 43 kDa). I would like to improve my skills with other calculation approaches since I've only been using ARIA with CNS/XPLOR so far. The assignment sessions would help me a lot since I'm only just now embarking on assigning my 43 kDa protein. Knowledge of structure validation of NMR structures is also an important topic which at present is a bit scarce in our lab.
(60) Anton Zavialov (SLU) 22 June, 2003 Visit to Dr. Vladimir M. Tischenko (or Tishchenko), Laboratory of Modern Problem of Biology at the Institute of Biological Instrumentation of the Russian Academy of Sciences 18 June - 18 July, 2003
Our structural study on assembly system of F1 capsular antigen of Y. pestis resulted in a model for chaperone-mediating folding, assembly, and chaperone recycling (Zavialov et al. 2003, Cell 113:587-596). According to the model, periplasmic chaperone traps folding intermediate of structural subunit of F1 fibers. Folding energy plays a key role in both chaperone-subunit binding and chaperone-subunit dissociation upon assembly. Chaperone-subunit binding is accompanied by partial subunit folding. The folding is completed during chaperone-subunit dissociation and assembly. This structure-based model requires experimental confirmation with characterization of the thermodynamics and kinetics of the process. We therefore plan to investigate the process using an array of biophysical and biochemical methods. I am going to visit laboratory of Dr. Vladimir M. Tischenko to study several novel biophysical and biochemical methods, which will be applied for our research. We also are interested to establish in future a small laboratory in our floor, where we would be able to perform experiments with differential adiabatic scanning microcalorimetry (DASM) measurements.
(59) Alina Castell (Uppsala University) 10 May, 2003 Attend the 7th International School on the crystallography of Biological Macromolecules in Como 9-16 May, 2003
I started as a PhD student in January this year and I have only attended one crystallography course so far. This course will provide me with basic and new knowledge that I badly need for my further research as a structural biologist. The concept of a one week course like this fits me better than attending several short courses in a wide range of time.
(58) Annette Roos (Uppsala University) 10 May, 2003 Attend the 7th International School on the crystallography of Biological Macromolecules in Como 9-16 May, 2003
The Crystallography School in Como focuses on methods and applications of X-ray crystallography emphasising the latest developments in the field. Lectures will deal with the different parts of the process of proceeding from protein expression to published structure with some participants describing their own structures. The School has been recommended to me by my supervisor as an excellent place to learn about new developments, get new ideas for my current research and to meet people from different structural biology laboratories.
(57) Emma Jakobsson (Uppsala University) 10 May, 2003 Attend the 7th International School on the crystallography of Biological Macromolecules in Como 9-16 May, 2003
At the School held in Como methods in protein crystallography ranging from expression and crystallisation to map modification and interpretation will be discussed and it is a good possibility to hear about the latest development in the field. Further it is time for me to think of what to do after my PhD and this will be a great opportunity to meet and talk to researchers from other laboratories and get ideas about possible postdocs.
(56) Lena Henriksson (Uppsala University) 10 May, 2003 Attend the 7th International School on the crystallography of Biological Macromolecules in Como 9-16 May, 2003
During my Phd studies I will purify and crystallize M.tuberculosis proteins with the aim of solving their structures. The International School on the Crystallography of Biological Macromolecules will be my first international course. I am looking forward to holding my first poster presentation and learning more about the crystallography field.
(55) Annette Roos (Uppsala University) 25 March, 2003 Attend "RapiData 2003" at NSLS 5-14 April, 2003
I've been accepted to go to the RapiData course at Brookhaven National Laboratory as an experimentalist. The course will cover methods for diffraction data collection, especially at synchrotron radiation sources. MAD phasing is one of the main topics and I will be taking some of my own crystals soaked in platinum to use as a MAD data collection example.
(54) Nicusor Timneanu (Uppsala University) 4 March, 2003 Attend the Summer School on "ULTRAFAST X-RAY SCIENCE" in Cargese, France 13-19 July, 2003
I am a theoretical physicist converting to biology/biophysics, with the aim to exploit X-ray lasers in structural studies. This trip will give me the opportunity to learn more about the key issues in ultrafast X-ray sources and their applications. Experts from the best laboratories in the field will contribute to this school and I believe that contact with them will be beneficial. I hope to learn more about the X-ray instrumentation and techniques, as well as their applications. This knowledge will provide a solid background for my project and for my future theoretical calculations on the impact of X-ray in biology.
(53) Gösta Huldt (Uppsala University) 4 March, 2003 Attend the Summer School on "ULTRAFAST X-RAY SCIENCE" in Cargese, France 13-19 July, 2003
There are very few opportunities to study this field directly in a school and a course. In most cases, existing courses may be relevant to a certain extent but have their focus on another area, such as crystallography, tomography or signal processing. The Corsica school would be an opportunity to meet others interested in this field, and learn about the current ideas and techniques.
(52) Carl Caleman (Uppsala University) 4 March, 2003 Attend the Summer School on "ULTRAFAST X-RAY SCIENCE" in Cargese, France 13-19 July, 2003
The summer school is focusing on exact the things that my project is about; and there are no possibilities to take part in any such courses here in Uppsala. I hope to get to know more about the physics behind the accelerators and the technique to use the sources to preform future experements at SPPS and DASEY
(51) Magnus Bergh (Uppsala University) 4 March, 2003 Attend the Summer School on "ULTRAFAST X-RAY SCIENCE" in Cargese, France 13-19 July, 2003
My research project deals with applications of the free electron laser (x-ray region) in biology. The Summer School will cover the latest research in this field, and also give me the opportunity to meet a lot of people who are involved in the XFEL project. At this stage I'm working with simulations (in GROMACS) of a molecule bombarded with an XFEL beam. Details of the beam performance will be crucial for a realistic model.
(50) Stina Lundgren (KI) 3 March, 2003 Attend "RapiData 2003" at NSLS 5-14 April, 2003
To be able to take a project from the crystallization of a protein to the final structure. Today I lack the practical experience of data collection and phasing, something I expect to learn at the course.
(49) Peter Holm (LU) 20 February, 2003 17 June - 12 July, 2003
Microsomal Glutathione Transferase 1 (MGST1) primarily using electron crystallography. Under certain conditions, pure solubilized MGST1 forms well ordered 2-D crystals following reconstitution with phospholipids. These two-dimensional crystals are suitable for analysis of the three-dimensional structure of the protein by electron crystallographic techniques. Our aim is to obtain a near-atomic resolution 3-D map of MGST1. We have during several journeys collected image and electron diffraction data on the 300kV liquid helium stage electron microscope equipped with a field-emission gun in Professor Yoshinori Fujiyoshi's lab in Kyoto, Japan. These data from two different crystal symmetries have been processed and rendered maps at 6 Å resolution [Schmidt-Krey et al (2000), Holm et al (2002)]. To increase the resolution further to 3-4 Å we need to extend the data sets and improve our image processing procedures. The former is continuously performed by crystallization and data collection. The latter however, requires input and discussion from external sources. To improve our procedures for data processing a visit to Dr Vinzenz Unger's lab at Yale University, New Haven, USA (http://www.mbb.yale.edu/fl/fl_v_unger.htm) will be performed in June this year. Dr Unger's excellence and critical reviewing of results within the field of Cryo-EM data processing is well acknowledged. In conjunction with the stay at Yale University, the Gordon Research Conference on Three-Dimensional Electron Microscopy in New London, New Hampshire, USA (http://www.grc.org/programs/2003/3d.htm) will be visited to further extend my understanding of the field.
(48) Anna Jansson (KI) 13 February, 2003 To attend the Seventh International School on the Crystallography of Biological Macromolecules in Como, Italy 10-15 May, 2003
I am doing my PhD in the field Structural Biology and the main focus of my PhD studies is to use crystallography as a tool for studying proteins alone and in complex with substrates/inhibitors and to by additional methods as mutagenisis and biochemical analysis elucidate their functions. In my project I work with some proteins involved in the production of anthracyclines, natural products commonly used in cancer treatment. Many of them have however been shown to exhert cardiotoxic sideffects and it is therefore important to find new potential drugs. Novel antibiotics have been produced by transferring genes between different Streptomyces species. I am studying enzymes responsible for the production of some of these new products. I have recently been solving the structures of two of these enzymes and the results of this research are as manuscripts right now. The emphasis of this course is focused on that the participants will tell about their own research and I would be very glad if I would get the chance to talk about my newest results. Also, the content of the course is closely related to my own area of research and it would be a great opportunity to get updated on the newest thing going on in the area of crystallography. Since I'm just having a little bit more then one year left on PhD studies it would also be useful for me to meet people from other laboratories and to start thinking about were I would like to do a postdoc. I have heard many good things about the course from people that have been there previoulsy and therefore I would be very happy to get the opportunity to attend this course.
(47) Sefano Ricagno (KI) 3 February, 2003 Attend the "RapiData" course at NSLS and visit to collaborating laboratory at University of Florida 6-16 April, 2003
The course has been suggested me by several people as a very useful experience where a lot of new skills could be obtained. About the second part of my trip it is important especially to discuss some issues about how to proceed in the research.
(46) Magnus Bergh (Uppsala University) 9 January, 2003 Visit Stanford LAC and the SPPS facility February-March, 2003
PREPARATIONS FOR THE FIRST EXPERIMENTS AT SPPS AT STANFORD To be able to perform future experiments at SPPS (in May), We need to be there to learn about the equipment. Since the facility is new, it is a must to go there in advance to see what preparations we have to do, and to get to know the beamline set-up. This is something that can not be done from the office at BMC. My hopes are that after this visit to SPPS I will know what has to be done to prepare the experiments and that I will get to know the people at the facility and learn about its features from them.
(45) Carl Caleman (Uppsala University) 9 January, 2002 Visit Stanford LAC and the SPPS facility February-March, 2003
PREPARATIONS FOR THE FIRST EXPERIMENTS AT SPPS AT STANFORD

To be able to perform future experiments at SPPS (in May), We need to be there to learn about the equipment. Since the facility is new, it is a must to go there in advance to see what preparations we have to do, and to get to know the beamline set-up. This is something that can not be done from the office at BMC. My hopes are that I after this visit to SPPS will know what has to be done to prepare the experiments and that I will get to know the people at the facility and learn about its features from them.

(44) Tobias Karlberg (Lund University) 9 January, 2003 To visit professor Mark O'Brien's lab at the State Univeristy of New York in Buffalo, NY to purify protein. 5 February - 28 March, 2003
The lab of prof. Mark O'Brien at The State University of New York in Buffalo, have the expertise in working with the iron-response regulator (irr). irr is believed to have a regulatory role in heme biosynthesis and is associated with ferrochelatase. The work I intend to do is purification of irr for later attempts to obtain the crystal structure of the complex irr-ferrochelatase.
(43) Ulrika Magnusson (Uppsala University) 9 January, 2003 Attend the CCP4 study weekend in York 2-6 January, 2002
The CCP4 program package is used a lot for data processing and structure solving. I would like to learn more about it, especially phasing since I don't have much experience in that field.
(42) Annette Roos (Uppsala University) 10 December, 2002 Attend the CCP4 study weekend in York 2-5 January, 2002
I have recently collected a native data set for crystals of an M. tuberculosis protein I'm working with. Going to the CCP4 workshop will give me a lot of useful information about phasing, refinement, and how to use the CCP4 software, which will help me to solve the structure.
(41) Martin Welin (SLU) 28 November, 2002 Attend the CCP4 study weekend in York 2-5 January, 2002
This study-weekend at the University of York will not only give me an overview of CCP4 but also information about how to make derivatives, data collection, twinning, refinement and phasing.

2002

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(40) Linda Öster (SLU) 27 November, 2002 Lab visit to purify protein at Chris Schofields lab, Oxford Centre for Molecular Sciences, University of Oxford 2-8 December, 2002
I want to visit our collaborators in Chris Schofields lab in Oxford to learn a purification method for a protein involved in the clavulanic acid biosynthesis.
(39) Li Xing (KI) 27 November, 2002 Cryo-EM data-collection in the lab of Prof. Yoshinori Fujiyoshi, department of Biophysics, Kyoto University 14-22 December, 2002
The purpose of the trip is to collect high quality cryo-EM data and to gain experience in working with liquid helium, 300kV FEG microscope in Kyoto lab. Under the supervision of Prof. Yoshinori Fujiyoshi, the key developer of a new-generation microscope, I believe the improved quality of the image data would be helpful to reveal viral capsid arrangement and molecular interaction between the virus and antibody at atomic level.
(38) Ola Hansson (SLU) 21 November, 2002 EMBO Practical course on Protein Expression, Purification and Crystallisation (PEPC3) in Hamburg. 3-12 December, 2002
I will attend the EMBO Practical course on Protein Expression, Purification and Crystallisation (PEPC3) in Hamburg. The course, intended for students in the early stages of their research career, will cover over-expression of proteins in prokaryotic and eukaryotic systems, protein purification and characterisation, crystallization techniques and determination of crystal quality.
(37) Ola Hansson (SLU) 26 August, 2002 Spetses Summer school on Protein folding and protein ligand interactions 19-28 September, 2002
I'm currently trying to improve the yield and purity of a glycosyltransferase to be able to set up crystallization trials. The crystal structure of this enzyme will help the design of new inhibitors that are being synthesized by our collaborators. This course on protein folding and protein ligand interactions will increase my knowledge of protein chemistry, which will be helpful during this and upcoming projects.
(36) Martin Welin (SLU) 29 May, 2002 Spetses Summer school on Protein folding and protein ligand interactions 19-28 September, 2002
I have recently started with my Ph. D. at the Department of Molecular Biology (SLU). I think this course can provide me with the latest findings in the area of protein folding and protein ligand interactions.
(35) Anna Jansson (KI) 24 April, 2002 33rd Crystallographic Course at the International School of Crystallography in Erice 23 May-3 June, 2002
I'm doing my PhD in structural biology and the main interest of the group is to use crystallography as a tool for studying proteins alone and in complex with substrates/inhibitors.
In my project I work with some proteins involved in the production of new anthracycline antibiotics. These hybrid antibiotics are produced by transferring genes from one Streptomyces bacteria strain to another. Some of them have been shown to exhert cytotoxic activity against mouse lekemia cellines.
More knowledge about how to decide and pick out target proteins and then how to design the appropiate drugs to inhibit or activate thoose proteins would be valuble for me in my research since it's something I'm going to work with sooner or later. Therefore I would be very happy to get the opportunity to be in this course.
(34) Tobias Karlberg (LU) 24 April, 2002 The "BBSRC Workshop on Phasing and Refinement" 7-14 April, 2002, York (UK) 6-14 April, 2002
I find the topics of the workshop both interesting and useful. I have some knowledge however not extensive, I have used XDS, CSN and O for molecular replacement, refinement and model building. Now, I would like to expand my knowledge to other phasing methods and refinement programs. The course in York would be ideal for this purpose.
(33) Malin Uppsten (SLU) 29 March, 2002 Workshop on twinning and the CRYSTALS program in connection to the XIX Congress of the International Union of Crystallography 5-16 August, 2002
My research project concerns structural investigations of ribonucleotide reductases and one of the most interesting things right now is the crystals of the holoenzyme. No structure of any holoenzyme ribonucleotide reductase has been solved so far but we have managed to get crystals of the holoenzyme from Salmonella typhimurium. Data has been collected to 4 . The problem now is that the data is twinned with a twinfactor of app 0.4 and we have not been able to solve the complex structure yet.
I am going to the XIX Congress of the International Union of Crystallography and it would be of great help for me to take part of the twinning workshop. My knowledge in twinning is limited and this is a great opportunity to learn more. It will hopefully help us to solve the complex structure and it would certainly broaden my knowledge in crystallography.
(32) Wimal Ubhayasekera (SLU) 9 May, 2002 Gordon Research Conference on Diffraction Methods in Structural Biology, and the laboratory of Dr Specht 10-20 July, 2002
The diffraction methods GRC is very important in my professional development, because I can discuss with most of the "key figures" in protein crystallography. This can motivate me in this field as well as I get to know the experts in this area. I have very basic knowledge in diffraction methods and I would like to get to know the subject as well as the people involved in it. So, this GRC will be one of the best places for that.
The visit to Boston University will make me the possibility to bring some protein to home lab. We have been discussing for a long time for working on structural studies of proteins active on chitin. This will help me to know more about these proteins and will strengthen our collaboration.
(31) Anna Suarez Larsson (Uppsala University) 12 March, 2002 RapiData 2002 workshop 20-28 April, 2002
Collecting data at synchrotrons is often made under stressed condition. It's very important to have as good basic knowledge as possible to make the correct decisions. I think this course can improve my ability to collect good diffraction data in a way that is useful for my PhD but also for other people in the lab that I'll be collecting data for or together with. According to colleagues in the lab that participated last year it's a well organized and nice course.
(30) Gisela Larsson (SU) 6 March, 2002 RapiData 2002 workshop 20-28 April, 2002
As I am the only crystallographer in our group, it is important for me to gain as much knowledge and training as possible in data collection strategies and data treatment. I already have some experience in collecting data at the ESRF, Grenoble, which I have done together with our collaborators. This course would make me more independent, and also more time efficient, on my future synchrotron visits.
(29) Linda Öster (SLU) 14 February, 2002 RapiData 2002 workshop 20-28 April, 2002
Since I have been working in the field of crystallography for two years now I feel I start to have have some experience but I also realise there is still very much to learn. This course would give me the perfect opportunity to learn more about collecting synchrotron data and solving structures. The course is a mixture of lectures and a practical part where you collect and work with your own data with experienced people around all the time. I am sure this would help me a lot in my future work.

2001

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(28) Karin Petersson (LU) 22 November, 2001 Prof. Roy Mariuzza at the University of Maryland Biotechnology Institute in Rockville, Maryland January, 2002
I am going to a conference in Keystone, Colorado in January and I am planning on going to visit the laboratory in Rockville afterwards. I have received scholarships for the trip to Keystone and I am now applying for scholarships to be able to go and visit Prof. Mariuzza's laboratory. I will stay a few days to learn how they do their TCR expression and also possibly present my newly published structure of SEH and MHC class II in complex. Dr. Eric Sundberg, who is a post-doc in the lab and recently visited us in Lund, has kindly asked me to come over and visit their laboratory.
(27) Andreas Karlsson (SLU) 26 October, 2001 University of Iowa (S. Ramaswamy and D. Gibson) November-December, 2001
The purose of my stay in Iowa is to learn more about the enzyme system I am working on (Ring Hydroxylating Dioxygenases), by using the expertise and equipment provided in Profs. Gibson and Ramaswamy's labs.
(26) Peter Holm (KI) 15 October, 2001 EM data-collection in the lab of Prof. Yoshinori Fujiyoshi in Kyoto October/November 2001
My research project is structural analysis of the small membrane protein Microsomal Glutathione Transferase 1 (MGST1) using Electron Crystallography. Under certain conditions, pure solubilized MGST1 forms well ordered 2-D crystals following reconstitution with phospholipids. These two-dimensional crystals are suitable for analysis of the three-dimensional structure of the protein by electron crystallographic techniques. Our aim is to obtain a 3-D map of MGST1 at atomic resolution. In order to achieve that it is important to collect the data in a liquid helium stage 300kV field-emission gun equipped electron microscope. This type of microscope is very stable and minimizes the radiation damage caused by the beam on the specimen.

We have collaboration with Professor Yoshinori Fujiyoshi's lab in Kyoto, Japan, where such a microscope is available. Since this microscope is state-of-the-art equipment in the field of biological electron crystallography it is necessary for me to obtain knowledge of how to operate such a microscope. Since we recently have managed to prepare well ordered 2-D crystals it is would be very suitable to bring those along to Kyoto to collect data and learn the technique of handling a He (l) electron microscope. We are interested in collecting tilted electron diffraction data. The period of time required for such a task would be approximately 2-3 weeks ranging from 24/10-2001 to 11/11-2001.

(25) Martin Högbom (SU) 24 September, 2001 Visit to the high-throughput structural genomics laboratory in Toronto, Canada, managed by Aled Edwards t.b.a.
1. To get hands-on knowledge of the methods they use for the entire high-throughput process from gene to structure. As they are a high-throughput lab that is up and running they have solutions to the technical problems associated, these are important for us to "import".

2. To initiate a joint project with them to identify metalloproteins from their expressed and purified protein samples using chemical analysis. This information will then be used to see if we can identify new metal-binding motifs in the sequences of these proteins and, of course, the ultimate goal is to solve the structures.

(24) Lars Haag (KI) 14 August, 2001 Hands-on Cryo-EM workshop in Eindhoven (NL) 2001-08-27 - 2001-08-31
The workshop will cover both theoretical and practical such as A. Steven (NIH), M. van Heel (UK) and W. Chiu (US). It will give me a chance to look at and practically handle their latest microscopes, ancillary equipment and of course talk the manufacturers as well the scientists. It will also give me an opportunity to collect data using today's most advanced microscopes.
(23) Willem van de Beek (KI) 6 August, 2001 Hands-on Cryo-EM workshop in Eindhoven (NL) 2001-08-27 - 2001-08-31
It is of great importance for me to follow this '7th Cryo Electron Microscopy Workshop' from the Dutch Society for Microscopy, FEI/Electron Optics and Leica in Eindhoven from august 27th up to august 31st. Not only will I learn the basics of CryoEM, since I am still a novice in this area, but I will also be updated to latest state of the art Cryo EM techniques in the field. This course covers aspects in both the theory and the practice, see http://www.feicompany.com/eng/pdf/cryo_workshop.pdf, provided by the leading scientists in the field and the complete lab of the manufacture, respectively.
(22) Li Xing (KI) 13 June, 2001 Hands-on Cryo-EM workshop in Eindhoven (NL) 2001-08-27 - 2001-08-31
This year, the workshop will be held at FEI/Electron Optics Application Laboratory with its wide range of essential equipment, including the latest microscopy and ancillary equipment. Thus, this workshop would provide me a nice opportunity to broader my knowledge on Cryo-electron microscopy and benefit my thesis work very much.
(21) Anna Suárez Larsson (UU) 28 May, 2001 EMBO course on Anomalous Scattering in Macromolecular Crystal Structure Solution at the ESRF in Grenoble, France 2001-06-19 - 2001-06-23
The course would fit perfectly at this stage of my project and give me valuable knowledge for future work with crystallography. The course includes both lectures and practical sessions. In the practical part we will learn absorption edge scanning, MAD/SAD data collection, processing, and structure solution techniques.
(20) Wimal Ubhayasekera (SLU) 28 May, 2001 2nd Summer school of the European Chitin Society "EUCHIS" on Chitin/chitosan enzymology in Ulm, Germany 2001-09-30 - 2001-10-06
The course will be dealing with chitin/chitosan degradation, synthesis and some structural studies of chitinases. As I have limited knowledge on chitin/chitosan and its synthesis and degradation, I think this course will give me a better knowledge to understand the importance of the above-mentioned enzymes. Therefore, I think that the intended course on chitin/chitosan will be very useful in my work.
(19) Patrik Johansson (UU) 21 February, 2001 "Higher European research Course for Users of Large Experimental Systems (HERCULES)" 2001-03-04 - 2001-04-11
Due to the nature of my project I am much interested in attending the course to learn more about synchrotron radiation and to get practical experience of data collection.
(18) Wimal Ubhayasekera (SLU) 21 February, 2001 CCP4 Refinement Course and CCP4 Molecular Replacement Study Weekend 2001-01
As I am in the begining of my PhD studies, and started refining structures, I need to know how to handle refinement programs.
(17) Rosmarie Friemann (SLU) 26 January, 2001 "Higher European research Course for Users of Large Experimental Systems (HERCULES)" 2001-03-04 - 2001-04-11
This course would give me a great opportunity to acquire knowledge and training in fields such as MAD data collection, EXAFS, small angle scattering, cryocrystallography, microdiffractometer, which is very essential for my Ph.D. education.
(16) Emma Jakobsson (UU) 23 January, 2001 "Rapid Data Collection and Structure Solving at the NSLS: A Practical Course in Macromolecular X-Ray Diffraction Measurement" 2001-04-22 - 2001-04-27
So far I have only worked on a project involving solving structures of protein-ligand complexes where the structure of the apoenzyme is already known. Now I have crystals of a protein where the structure is not known yet and I need to do some "real" crystallography. This course would come in at a very good time of my PhD, I have been collecting some data and worked with the datasets but now I need to get more training to be able to handle my projects. Today you can collect data and run many programs without really understanding the underlying theory and what you are actually doing. My goal is to get a more profound understanding of crystallography, both the theory and the practical part, e.g. how to judge the diffraction pattern in order to be able to make the right decisions at the synchrotron, get the most out of problematic datasets and interpret log-files to spot potential problems.
(15) Anna Jansson (KI) 19 January, 2001 "Rapid Data Collection and Structure Solving at the NSLS: A Practical Course in Macromolecular X-Ray Diffraction Measurement" 2001-04-22 - 2001-04-27
I would like to attend this course since I'm just in the process of solving a structure myself! The knowledge I will get out from the course will hopefully help me a lot in my future research. It would be great for me to meet other researchers in the same field, share the problems that I've been having during my research project, get tips and come up with new ideas and plans! I'm also going to share my experiences and knowledge with my lab when I come home.
(14) Jenny Berglund (SLU) 19 January, 2001 "Rapid Data Collection and Structure Solving at the NSLS: A Practical Course in Macromolecular X-Ray Diffraction Measurement" 2001-04-22 - 2001-04-27
Both my major projects are in the stage where crystals are starting to come. I will therefor soon be travelling to a synchrotron to collect data. This course would give me a practical head-start, and provide useful theoretical knowledge I will very soon need in order to progress with my project. I feel that this kind of course at exactly this stage of my project will considerably speed up later work and increase my theoretical understanding of structure solving. If I don't get support I won't be able to attend the course.

2000

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(13) Gergely Katona (CUT) 11 December, 2000 Protein Crystallography course in Copenhagen 2001-01-15 - 2001-02-02
To acquire a solid theoretical background in crystallography and to learn state-of-the-art techniques
(12) Jenny Sandmark (KI) 30 November, 2000 Laboratory of Jack Kirschis at Berkeley t.b.a.
To learn the methodology of an activity assay for 7,8-diaminopelargonic acid synthase and to get enough biochemical information of the wild type and mutant enzyme to continue kinetic crystallography in the home laboratory.
(11) Karl Edman (UU) 12 January, 2000 Laboratory of Professor Ehud Landau in Dallas March, 2000
To learn about the latest developments in the lipidic cubic phase approach for crystallization
(10) Jeff Abramson (UU) 12 January, 2000 Laboratory of Professor Ehud Landau in Dallas March, 2000
To learn about the latest developments in the lipidic cubic phase approach for crystallization

1999

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(9) Cecilia Svensson (GU) 12 October, 1999 F. Titgemeyer & M. Dahl, Institute for Microbiology, University of Erlangen-Nürnberg, Germany August, 1999
To learn how to purify several sugar-binding proteins and to perform activity assays
(8) Andreas Muranyi (LU) 21 September, 1999 EMBL Heidelberg 12 - 19 September, 1999
EMBO Practical Course on Structure Determination of Biological Macromolecules by Solution NMR
(7) Emma Jakobsson (UU) 18 February, 1999 Professor J. C. Shih, University of Southern California, Los Angeles 8-19 March, 1999
To learn how to express and purify MAO-A in baculovirus
(6) Elinor Eriksson (SLU) 20 January, 1999 Washington University School of Medicine, St. Louis, Missouri January-March, 1999
To learn how to express and purify chaperone-subunit protein complexes of type 1 pili

1998

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(5) Henrik Hansson (KI) 20 February, 1998 3rd European Conference on Stable Isotope Aided NMR of Biomolecules 5-7 April, 1998
To gain expertise on the synthesis of labeled biomolecules and application of them in NMR
(4) Ingeborg Schmidt-Krey (KI) 6 February, 1998 Professor Fujiyoshi, International Institute for Advanced Research, Kyoto 1998
To collect data, and to acquire data-processing skills
(3) Martin Laurberg (LU) 2 February, 1998 HERCULES "Biomolecular Structure and Dymamics" course, Grenoble 22 February - 3 April, 1998
To gain structure determination and synchrotron beam-line expertise
(2) Jenny Cromsigt (UmU) 28 January, 1998 3rd European Conference on Stable Isotope Aided NMR of Biomolecules 5-7 April, 1998
To gain expertise on the synthesis of labeled biomolecules and application of them in NMR

1997

Student Date awarded Laboratory/Course Period
Purpose/Motivation
(1) Magnus Helgstrand (KI) 22 December, 1997 Carlsberg Laboratory, Copenhagen (Flemming Poulsen) 13-14 January, 1998
To gain hands-on experience with the NMR assignment software Pronto


SBNet Latest update at 29 March, 2004.