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Protein expression in fungal hosts

 

The entire block will take place at Biocenter!

 

Nov 29th, Tuesday

09:00-11.00 Lecture: Introduction to protein purificatio systems and strategies (Saeid) Mar-77

11.00-12:00 Distribution of group task about protein purifications

12:00-13:00 Lunch

13:00-17:00 Time for preparation of group tasks

 

Nov 30th, Wednesday

09:00-12.00 Visit at GH Healthcare in Uppsala

12:00-14:00 Lunch

14:00-17:00 Wet Lab: Preparation of pre-culture media for fungal expression cultures (Saeid) BioC:Lab

 

Dec 1st, Thursday

10:00-11.00 Lecture: Expression of proteins in Pichia pastoris (Mats ) Mar-71

11:00-12.00 Introduction to protein expression in fungal hosts (Mats) Mar-71

12:00-13:00 Lunch

13:00-16:00 Wet lab: Preparation of culture media for big fungal protein expressions, start of small pre cultures (Mats) BioC:Lab

 

Dec 2nd, Friday

10:00-12:00 Lecture: Carbohydrate active enzymes (Jerry) BioC-C212

12:00-13:00 Lunch

13:00-17:00 Wet Lab: Start of big fungal protein expression cultures (Mats) BioC:Lab

 

Dec 5th, Monday

09:00-9:30 Introduction to protein isolation and purification (Mats) BioC-C216

09:30-12:00 Wet Lab: Start purification of expressed proteins (Saeid) BioC:Lab

12:00-13:00 Lunch

13:00-17:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

 

Dec 6th, Tuesday

9:00-12:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

12:00-13:00 Lunch

13:00-17:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

 

Dec 7th, Wednesday

9:00-12:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

12:00-13:00 Lunch

13:00-14:00 Lecture: Industrial enzymes and their optimization (Mats) BioC-C212

14:00-17:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

 

Dec 8th, Thursday

9:00-12:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

12:00-13:00 Lunch

13:00-17:00 Wet Lab: Continue with protein purifications (Saeid) BioC:Lab

 

Dec 9th, Friday

09:00-12:00 Wet Lab: Harvest of H. jecorina cultures. BioC:Lab

12:00-13:00 Lunch

13:00-17:00 Start to characterize the expressed and purifyed proteins

 Gel electrophoresis and spectrometric characterization of expressed and purified proteins

 

 

Dec 12th, Monday

09:00-10:00 Lecture: spectrometric characterization of enzyme activity (Mats) BioC-C213

10:00-12:00 Wet Lab: Characterize enzyme activity of purified and expressed proteins (Nils) BioC:Lab

12:00-13:00 Lunch

13:00-17:00 Wet Lab: Characterize enzyme activity of purified and expressed proteins (Nils) BioC:Lab

 

Dec 13th, Tuesday

09:00-11:00 Lecture Biophysical characterizations of enzymes (TorLeif) BioC-A241

11:00-13:30 Lunch

13:30-17:00 Visit at Phadia in Uppsala

 

Dec 14st, Wednesday

10:00-11:00 Introduction to oral presentations and written Lab-repports on protein expression and protein purification part (Saeid) AU20

11:00- ?      Write group Lab reports on fungal protein expression Lab part

 

Dec 15th, Thirsday

Write group Lab reports on fungal protein expression Lab part

 

Dec 16th, Friday

09:00-11:00 Oral presentation of results from fungal expression and protein expression Lab part BioC-C216

11:00-12:00 Summary and evaluation of fungal protein expression and protein purification part of the course. Questions regarding written Lab repport. BioC-C216

 

 

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