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Course literature

 

 

Books (do not purchase, they are available at the department) :

 

PROTEINS: Structure and Molecular Properties

T. E. Creighton, 2nd ed., W. H. Freeman and co,1993

 

PROTEIN PURIFICATION: Principles and Practice

R. K. Scopes, 3rd ed., Springer, 1994

 

PROTEIN PURIFICATION: Principles, High-Resolution Methods,

and Applications

J.-C. Janson and L. Ryden, 2 end ed., J. Wiley and Sons, Inc., 1995

 

 

Articles and Useful Handbooks:

 

Protein expression

 

Advanced genetic strategies for recombinant protein expression in Escherichia coli

 

pBAD TOPO® TA Expression manual

 

pET Directional TOPO® Expression manual

 

Protein purification

 

Affinity Chromatography Handbook: Principles and methods

 

Gel Filtration: Principles and methods

 

Ion Exchange Chromatography: Principles and methods

 

Hydrophobic Interaction and Reversed Phase Chromatography:

Principles and Methods

 

High-level expression and purification of 6xHis-tagged proteins

 

Binding assays

 

Quantitative Analysis of Protein–Protein Interactions

 

Protein stability

 

Circular Dichroism of Protein-Folding Intermediates

 

 

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