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Course literature



Books (do not purchase, they are available at the department) :


PROTEINS: Structure and Molecular Properties

T. E. Creighton, 2nd ed., W. H. Freeman and co,1993


PROTEIN PURIFICATION: Principles and Practice

R. K. Scopes, 3rd ed., Springer, 1994


PROTEIN PURIFICATION: Principles, High-Resolution Methods,

and Applications

J.-C. Janson and L. Ryden, 2 end ed., J. Wiley and Sons, Inc., 1995



Articles and Useful Handbooks:


Protein expression


Advanced genetic strategies for recombinant protein expression in Escherichia coli


pBAD TOPO® TA Expression manual


pET Directional TOPO® Expression manual


Protein purification


Affinity Chromatography Handbook: Principles and methods


Gel Filtration: Principles and methods


Ion Exchange Chromatography: Principles and methods


Hydrophobic Interaction and Reversed Phase Chromatography:

Principles and Methods


High-level expression and purification of 6xHis-tagged proteins


Binding assays


Quantitative Analysis of Protein–Protein Interactions


Protein stability


Circular Dichroism of Protein-Folding Intermediates



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