Bke2 Biochemistry Lectures

Lecture 31: Cloning of DNA
Reading material: Horton, Chapter 24 in 2nd Ed; 23 in 3rd Ed (Stryer: Chapter 6)

Abstract: Recombinant DNA technology has revolutionized biochemistry. Some of the methods will be summarized:

Restriction enzymes cleave DNA specifically. DNA can be sequenced by using dideoxynucleotides. Plasmides and phages can be used for DNA cloning in bacteria. Genomic and cDNA libraries can be constructed. A particular gene can be amplified by the polymerase chain reaction (PCR).

Key concepts:
Restriction enzymes
Blotting techniques
The Sanger dideoxy method of DNA sequencing
Synthetic nucleotide synthesis
Vectors
Libraries
PCR
cDNA
Cloning

Links:
Take a look at these very nice figures and drawings from the Graphics Gallery at Access Excellence:
Example of Restriction Enzyme action (EcoRI)
Schematic drawing of EcoRI cutting DNA
Cutting and splicing DNA
Cloning into a Yeast Artificial Chromosome (YAC)
Inserting a DNA sample into a plasmid
Nucleic acid hybridization

Lecture index

[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35]