Bke2 Biochemistry Labs

Table suitable for Experiment 1 / Experiment 2.

Reaction nr	Substrate- conc (M)	Background absorbance (a)	Absorbance measured	DAbsorbance (corrected) (b)	v (M/min) (c)	1/S (M-1)	1/v (min/M)
1
2
3
4
5
6
7
8
9
Control

Notes

(a) Control is the background for reaction 9. The others may be calculated as:

Background (reaction nr) = Control absorbance * [S] (reaction nr) / [S] (Control)

(b) The reaction takes place in a 0.5 ml volume, whereas the absorbance is measured in a 1.0 ml volume. The volume corrected absorbance difference between start and stop is obtained by:

DAbsorbance (corrected) = 2 * (Absorbance measured - Background)

(c) The reaction velocity v is obtained by :

v (M/min) = DAbsorbance (corrected) / (18300 * Dt)

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